https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26728 Thu 24 Mar 2022 11:33:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30171 in vitro effects on the human lung cells BEAS-2B following exposure to and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to Corexit dispersants EC9500 and EC9527, Water Accommodated Fraction (WAF) -crude, WAF-9500 + Oil, and WAF-9527 + Oil. Cellular cytotoxicity to WAF-dispersed oil samples was observed at concentrations greater than 1000 ppm with over 70% of observed cellular death. At low concentration exposures (100 and 300 ppm) DNA damage was evidenced by the detection of single strand breaks (SSBs) and double strand breaks (DSBs) as measured by alkaline and neutral comet assay analyses. Immunoblot analyses of the phosphorylated histone H2A.X (ɣ-H2A.X) and tumor suppressor p53 protein confirmed activation of the DNA damage response due to the exposure-induced DNA breaks. Although, many xenobiotics interfere with DNA repair pathways, in vitro evaluation of the nucleotide excision repair (NER) and DSB repair pathways appear to be unaffected by the oil-dispersant mixtures tested. Overall, this study supports that oil-dispersant mixtures induce genotoxic effects in culture.]]> Sat 24 Mar 2018 07:26:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32133 in vitro can lead to cell death. At low concentration NaAsO₂ can induce autophagy and at high concentration it can induce apoptosis. The work presented in this poster shows the initial cellular response of human lung epithelial A549 cells to acute exposure to NaAsO₂ in vitro.]]> Mon 23 Sep 2019 12:35:47 AEST ]]>